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Objective To compare the toxicity of different extraction parts of Aconitum sinomontanum Nakai;To screen out"toxic sections"of Aconitum sinomontanum Nakai; To provide references for further study on toxicity components of Aconitum sinomontanum Nakai. Methods Systematic solvent method was used to extract the 95% ethanol extracts of Aconitum sinomontanum Nakai,and six different extraction fractions(petroleum ether,chloroform, ethyl acetate, butanol, alcohol and water) were obtained. Median lethal dose (LD50) and maximum dose method were used to conduct comparative study on acute toxicity of different extraction parts of Aconitum sinomontanum Nakai. Results Chloroform, water and butanol extractions in LD50of Aconitum sinomontanum Nakai were 89.65, 1805.40 and 24 409.41 mg/kg, and 95% confidence limits were 76.39~108.41, 1521.60~2240.00 and 20 422.54~24 246.95, respectively. The maximum dose of petroleum ether, ethyl acetate and alcohol extractions were 2686.01, 3108.13 and 28 376.21 mg/kg, respectively. Conclusion The maximum toxicity is the extracted section of chloroform, and the minimal toxicity is the extracted section of ethanol.
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Objective: To study the chemical constituents from the roots of Aconitum sinomontanum. Methods: Silica gel, Sephadex LH-20 column chromatography, high performance liquid chromatography, and other chromatographic techniques were used for separation and purification. The structures were elucidated by physiochemical methods and spectral data. Results: Five compounds were isolated from the 80% ethanol extract in the roots of A. sinomontanum, and their structures were identified as 6β,7β,8β,15α- tetrahydroxy-1α,14α,16β,18β-tetramethoxy-aconitan-19-en (1), delcosine (2), lepenine (3), napelline (4), and kirinine B (5). Conclusion: Compound 1 is a new compound named sinomontanum J. Compounds 2-5 are isolated from A. sinomontanum for the first time.
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OBJECTIVE To establish the HPLC multiple wavelength chromatographic fingerprints (MWCF) of different extracts of Aconitum sinomontanum Nakai (ASN) to clarify the attribution of the fingerprint peaks and their contribution to the acute toxicity. METHODS The experimental drugs (extracts of petroleum ether, chloroform, ethyl acetate, butanol, alcohol and water) were obtained by means of systematic solvent extraction from the 95% ethanol extract of ASN. The HPLC MWCF of different extracts of ASN were established by mean fingerprint method (MFM). The acute toxicity of different extracts in mice were carried out by measuring the median lethal dose (LD50) and maximum dose. The relationship between spectrum and toxicity was established by gray relational analysis. RESULTS The MWCF of different extracts of ASN were established. The acute toxicity of ASN was caused not only by lappacontine (LAP) and ranaconitine, the contribution of other diterpenoid alkaloids should not be neglected, and the contribution of different peaks to toxicity was ranked as (51, 38, 37, 35, 20) 3439323133. CONCLUSION The MWCF developed by MFM can maximally retain the fingerprint peaks, achieve fingerprint information maximization, and effectively improve fingerprint signal quality, thus providing a reference for the comprehensive quality evaluation of ASN. The relationship between the MWCF of different extracts and the acute toxicity is paralleled to some extent. And this will lay a foundation for the research of the toxicity mechanism of ASN.
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Objective: To set up an analysis method of fingerprints for analgesic and anti-inflammatory effective parts from crude and processed roots of Aconitum sinomontanum (AS), and to discuss the chemical composition changes after processing that enhance the analgesic and anti-inflammatory effect. Methods: HPLC gradient elution method was developed to establish fingerprints for 10 batches of chloroform extract from crude and processed the roots of AS in different areas. And the fingerprint were analyzed and compared by Chinese Materia Medica (CMM) Fingerprint Similarity Evaluation System (2012 edition). Results: The fingerprints of chloroform extract from crude and processed the roots of AS were set up by HPLC. The gained 3 and 15 common peaks from crude and processed roots of AS, respectively. processed product added 12 peaks, including 1 peak, 2 peak, 7 peak increase were significant, accounting for the new peak area of 72.3%-84.5%. And determination of lappacontine and ranaconitine of chloroform extract from crude and proceed products, after processing the content were reduced, ranaconitine content reduced to the original one-third. Conclusion: This method with good reproducibility, and strong characteristic, and could be used for the full quality evaluation of analgesic and anti-inflammatory effect parts from crude and processed the roots of AS. To provide scientific basis for elucidating the chemical substance base and processing principle of crude and processed roots of AS.
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Objective To provide herbalist basis for development of resource-related varieties through the study of herbal textual research of Aconitum sinomontanum Nakai, alias and source of production. Methods Description of Aconitum sinomontanum Nakai and its alias from the ancient herbal books was analyzed. Literature was searched to clarify modern research and conduct analysis. Results Ancient herbal textual records of Aconitum sinomontanum Nakai was not clear enough. The first records about Aconitum sinomontanum Nakai started from late Qing Dynasty, from which the source of production, medicinal parts, alias, harvest processing, efficacy and application of Aconitum sinomontanum Nakai were summed up. Conclusion Aconitum sinomontanum Nakai is the dry roots of Aconitum from Ranunculaceae, which has good medicinal prospects and development value, and can provide basis and guidance for the late clinical application and research.
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Objective To study the routine examination of Aconitum sinomontanum Nakai and determine the contents of lappacontine and ranaconitine; To provide basis for establishing the quality standard of Aconitum sinomontanum Nakai.Methods Aconitum sinomontanum Nakai were collected from different areas.A method of TLC was used for qualitative discrimination. The methods in the Chinese Pharmacopoeia were adopted for the determination of moisture content, ash content and extractives. Determination of lappacontine and ranaconitine were performed by HPLC. Results The TLC showed that the spots were clear and the separation was good. Individual provisional standards:the moisture,total ash and acid-insoluble ash content of Aconitum sinomontanum Nakai were not more than 11.0%, 12.0%, and 7.0%, respectively; water soluble and alcohol soluble extractives were not less than 18.2% and 10.6%,respectively.The content of ranaconitine and lappacontine in Aconitum sinomontanum Nakai were not less than 0.125% and 0.815%, respectively. Conclusion The method established by the study is accurate and reliable,and can be used for quality evaluation of Aconitum sinomontanum Nakai.
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Objective To study the routine examination of Aconitum sinomontanum Nakai and determine the contents of lappacontine and ranaconitine; To provide basis for establishing the quality standard of Aconitum sinomontanum Nakai.Methods Aconitum sinomontanum Nakai were collected from different areas.A method of TLC was used for qualitative discrimination. The methods in the Chinese Pharmacopoeia were adopted for the determination of moisture content, ash content and extractives. Determination of lappacontine and ranaconitine were performed by HPLC. Results The TLC showed that the spots were clear and the separation was good. Individual provisional standards:the moisture,total ash and acid-insoluble ash content of Aconitum sinomontanum Nakai were not more than 11.0%, 12.0%, and 7.0%, respectively; water soluble and alcohol soluble extractives were not less than 18.2% and 10.6%,respectively.The content of ranaconitine and lappacontine in Aconitum sinomontanum Nakai were not less than 0.125% and 0.815%, respectively. Conclusion The method established by the study is accurate and reliable,and can be used for quality evaluation of Aconitum sinomontanum Nakai.
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OBJECTIVE: To establish the fingerprint identification and assay for the quality analysis of processed Aconitum sinomontanum Nakai from different areas. METHODS: HPLC Gradient elution method was developed to establish the fingerprints for processed Aconitum sinomontanum Nakai, and the fingerprints were analyzed and compared by Chinese Materia Medica (CMM) Fingerprint Similarity Evaluation System (2012 edition), principal component analysis (PCA) and cluster analysis (CA). RESULTS: The common fingerprint for processed Aconitum sinomontanum Nakai fingerprints was established, and 18 common fingerprint peaks were identified. The similarity was greater than 0.90 among 10 batches of processed Aconitum sinomontanum Nakai medicinal herbs, and the contents of lappacontine and ranaconitine were determined. The samples from different areas could be classified into four groups, which reflected the quality characteristics of 10 batches of processed Aconitum sinomontanum Nakai from different areas. Four main components with cumulative contribution rate of 88.824% were selected by PCA, and seven chemical components were identified as the ones to determine the quality of processed Aconitum sinomontanum Nakai. CONCLUSION: This method, with good reproducibility and strong characteristics, can be used for the comprehensive quality evaluation of processed Aconitum sinomontanum Nakai.